Human PINK1 cDNA Clone in Mammalian Expression Vector
This is a PTEN Induced Putative Kinase 1 plasmid from OriGene - 6 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2710 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(6 references)
Catalog No. ABIN3385829
Supplier Product No.: sc108012
$803.88
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PINK1 cDNA Clone in Mammalian Expression Vector (ABIN3385829)
Gene
PINK1
(PTEN Induced Putative Kinase 1 (PINK1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc108012
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PINK1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2710 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "In Vitro Comparison of the Activity Requirements and Substrate Specificity of Human and Triboleum castaneum PINK1 Orthologues." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146083, (2016) (PubMed).
: "PINK1 kinase catalytic activity is regulated by phosphorylation on serines 228 and 402." in: The Journal of biological chemistry, Vol. 290, Issue 5, pp. 2798-811, (2015) (PubMed).
: "Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression." in: Neurobiology of disease, Vol. 62, pp. 426-40, (2013) (PubMed).
: "The kinase domain of mitochondrial PINK1 faces the cytoplasm." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 33, pp. 12022-7, (2008) (PubMed).
: "C-terminal truncation and Parkinson's disease-associated mutations down-regulate the protein serine/threonine kinase activity of PTEN-induced kinase-1." in: Human molecular genetics, Vol. 15, Issue 21, pp. 3251-62, (2006) (PubMed).
: "Wild-type PINK1 prevents basal and induced neuronal apoptosis, a protective effect abrogated by Parkinson disease-related mutations." in: The Journal of biological chemistry, Vol. 280, Issue 40, pp. 34025-32, (2005) (PubMed).
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- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
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Alternative Name
- PINK1
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Background
- This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_032409, NP_115785
Target
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