Human PRPF8 cDNA Clone in Mammalian Expression Vector
This is a PRP8 Pre-mRNA Processing Factor 8 plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 7140 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(4 references)
Catalog No. ABIN3386068
Supplier Product No.: sc116070
$1,990.89
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PRPF8 cDNA Clone in Mammalian Expression Vector (ABIN3386068)
Gene
PRPF8
(PRP8 Pre-mRNA Processing Factor 8 (PRPF8))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116070
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PRPF8 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 7140 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Splicing Factor Prp8 Interacts With NES(AR) and Regulates Androgen Receptor in Prostate Cancer Cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 29, Issue 12, pp. 1731-42, (2015) (PubMed).
: "The core spliceosome as target and effector of non-canonical ATM signalling." in: Nature, Vol. 523, Issue 7558, pp. 53-8, (2015) (PubMed).
: "Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with Prp8." in: PLoS pathogens, Vol. 10, Issue 6, pp. e1004199, (2014) (PubMed).
: "Metabolic profiling reveals PAFAH1B3 as a critical driver of breast cancer pathogenicity." in: Chemistry & biology, Vol. 21, Issue 7, pp. 831-40, (2014) (PubMed).
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- PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))
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Alternative Name
- PRPF8
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Background
- Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_006445, NP_006436
Target
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