Human PYCARD cDNA Clone in Mammalian Expression Vector
This is a PYD and CARD Domain Containing plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 730 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(3 references)
Catalog No. ABIN3386189
Supplier Product No.: sc115313
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PYCARD cDNA Clone in Mammalian Expression Vector (ABIN3386189)
Gene
PYCARD
(PYD and CARD Domain Containing (PYCARD))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc115313
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PYCARD is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 730 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Inflammasome activation has an important role in the development of spontaneous colitis." in: Mucosal immunology, Vol. 7, Issue 5, pp. 1139-50, (2014) (PubMed).
: "Gene-based vaccination and screening methods to develop monoclonal antibodies." in: Methods in molecular biology (Clifton, N.J.), Vol. 1121, pp. 337-46, (2014) (PubMed).
: "5-azacytidine reverses drug resistance in bladder cancer cells." in: Anticancer research, Vol. 31, Issue 11, pp. 3757-66, (2011) (PubMed).
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- PYCARD (PYD and CARD Domain Containing (PYCARD))
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Alternative Name
- PYCARD
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Background
- This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm, however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
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NCBI Accession
- NM_013258, NP_037390
Target
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