Human RHEB cDNA Clone in Mammalian Expression Vector
This is a Ras Homolog Enriched in Brain plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3386368
Supplier Product No.: sc116636
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human RHEB cDNA Clone in Mammalian Expression Vector (ABIN3386368)
Gene
RHEB
(Ras Homolog Enriched in Brain (RHEB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116636
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RHEB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "In vivo AAV1 transduction with hRheb(S16H) protects hippocampal neurons by BDNF production." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 3, pp. 445-55, (2015) (PubMed).
: "Formation of endothelial lumens requires a coordinated PKCepsilon-, Src-, Pak- and Raf-kinase-dependent signaling cascade downstream of Cdc42 activation." in: Journal of cell science, Vol. 122, Issue Pt 11, pp. 1812-22, (2009) (PubMed).
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: "In vivo AAV1 transduction with hRheb(S16H) protects hippocampal neurons by BDNF production." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 3, pp. 445-55, (2015) (PubMed).
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- RHEB (Ras Homolog Enriched in Brain (RHEB))
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Alternative Name
- RHEB
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Background
- This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_005614, NP_005605
Target
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