Human RIC3 cDNA Clone in Mammalian Expression Vector
This is a Resistance To Inhibitors of Cholinesterase 3 Homolog plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 4400 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3386375
Supplier Product No.: sc112180
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human RIC3 cDNA Clone in Mammalian Expression Vector (ABIN3386375)
Gene
RIC3
(Resistance To Inhibitors of Cholinesterase 3 Homolog (RIC3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc112180
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RIC3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4400 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "New quinoline derivatives as nicotinic receptor modulators." in: European journal of medicinal chemistry, Vol. 110, pp. 246-58, (2016) (PubMed).
: "Peptides from puff adder Bitis arietans venom, novel inhibitors of nicotinic acetylcholine receptors." in: Toxicon : official journal of the International Society on Toxinology, Vol. 121, pp. 70-76, (2016) (PubMed).
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: "New quinoline derivatives as nicotinic receptor modulators." in: European journal of medicinal chemistry, Vol. 110, pp. 246-58, (2016) (PubMed).
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- RIC3 (Resistance To Inhibitors of Cholinesterase 3 Homolog (RIC3))
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Alternative Name
- RIC3
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Background
- The protein encoded by this gene promotes functional expression of homomeric nicotinic acetylcholine receptors at the cell surface. It enhances currents generated by these receptors by expediting receptor transport to the cell surface and by increasing receptor number. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform a.
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NCBI Accession
- NM_024557, NP_078833
Target
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