Human SLC2A1 cDNA Clone in Mammalian Expression Vector
This is a Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2660 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(2 references)
Catalog No. ABIN3386773
Supplier Product No.: sc116011
$679.14
Plus shipping costs $50.00
10 μg
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Quick Overview for Human SLC2A1 cDNA Clone in Mammalian Expression Vector (ABIN3386773)
Gene
GLUT1 (SLC2A1)
(Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116011
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLC2A1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2660 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Overcoming 5-Fu resistance in human non-small cell lung cancer cells by the combination of 5-Fu and cisplatin through the inhibition of glucose metabolism." in: Tumour biology, Vol. 35, Issue 12, pp. 12305-15, (2014) (PubMed).
: "Overcoming 5-Fu resistance of colon cells through inhibition of Glut1 by the specific inhibitor WZB117." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 15, Issue 17, pp. 7037-41, (2014) (PubMed).
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- GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))
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Alternative Name
- SLC2A1
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Background
- This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013].
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NCBI Accession
- NM_006516, NP_006507
Target
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