Human SLC33A1 cDNA Clone in Mammalian Expression Vector
This is a Solute Carrier Family 33 Member 1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2640 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3386779
Supplier Product No.: sc117182
$506.88
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human SLC33A1 cDNA Clone in Mammalian Expression Vector (ABIN3386779)
Gene
SLC33A1
(Solute Carrier Family 33 Member 1 (SLC33A1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117182
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLC33A1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2640 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Deficient import of acetyl-CoA into the ER lumen causes neurodegeneration and propensity to infections, inflammation, and cancer." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 20, pp. 6772-89, (2014) (PubMed).
: "SLC33A1/AT-1 protein regulates the induction of autophagy downstream of IRE1/XBP1 pathway." in: The Journal of biological chemistry, Vol. 287, Issue 35, pp. 29921-30, (2012) (PubMed).
: "AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability." in: Journal of cell science, Vol. 123, Issue Pt 19, pp. 3378-88, (2010) (PubMed).
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- SLC33A1 (Solute Carrier Family 33 Member 1 (SLC33A1))
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Alternative Name
- SLC33A1
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Background
- The protein encoded by this gene is required for the formation of O-acetylated (Ac) gangliosides. The encoded protein is predicted to contain 6 to 10 transmembrane domains, and a leucine zipper motif in transmembrane domain III. Defects in this gene have been reported to cause spastic paraplegia autosomal dominant type 42 (SPG42) in one Chinese family, but not in similar patients of European descent. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010].Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.
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NCBI Accession
- NM_004733, NP_004724
Target
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