Human SNAI2 cDNA Clone in Mammalian Expression Vector
This is a Snail Family Zinc Finger 2 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(3 references)
Catalog No. ABIN3386857
Supplier Product No.: sc118205
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human SNAI2 cDNA Clone in Mammalian Expression Vector (ABIN3386857)
Gene
SLUG (SNAI2)
(Snail Family Zinc Finger 2 (SNAI2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118205
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SNAI2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1900 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "SNAILs promote G1 phase in selected cancer cells." in: International journal of oncology, Vol. 47, Issue 5, pp. 1863-73, (2015) (PubMed).
: "Endothelial progenitor cells undergo an endothelial-to-mesenchymal transition-like process mediated by TGFbetaRI." in: Cardiovascular research, Vol. 88, Issue 3, pp. 502-11, (2010) (PubMed).
: "Integrated proteomics and genomics analysis reveals a novel mesenchymal to epithelial reverting transition in leiomyosarcoma through regulation of slug." in: Molecular & cellular proteomics : MCP, Vol. 9, Issue 11, pp. 2405-13, (2010) (PubMed).
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- SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))
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Alternative Name
- SNAI2
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Background
- This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_003068, NP_003059
Target
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