Human STT3A cDNA Clone in Mammalian Expression Vector
This is a STT3, Subunit of The Oligosaccharyltransferase Complex, Homolog A plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2560 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3387048
Supplier Product No.: sc127610
$639.54
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human STT3A cDNA Clone in Mammalian Expression Vector (ABIN3387048)
Gene
STT3A
(STT3, Subunit of The Oligosaccharyltransferase Complex, Homolog A (STT3A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc127610
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human STT3A is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2560 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Mutations in STT3A and STT3B cause two congenital disorders of glycosylation." in: Human molecular genetics, Vol. 22, Issue 22, pp. 4638-45, (2013) (PubMed).
: "OST4 is a subunit of the mammalian oligosaccharyltransferase required for efficient N-glycosylation." in: Journal of cell science, Vol. 126, Issue Pt 12, pp. 2595-606, (2013) (PubMed).
: "Keratinocyte-associated protein 2 is a bona fide subunit of the mammalian oligosaccharyltransferase." in: Journal of cell science, Vol. 125, Issue Pt 1, pp. 220-32, (2012) (PubMed).
: "Diagnosis of suspicious thyroid nodules using four protein biomarkers." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 11 Pt 1, pp. 3311-8, (2006) (PubMed).
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- STT3A (STT3, Subunit of The Oligosaccharyltransferase Complex, Homolog A (STT3A))
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Alternative Name
- STT3A
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Background
- The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015].Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).
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NCBI Accession
- NM_152713, NP_689926
Target
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