Human TRIB3 cDNA Clone in Mammalian Expression Vector
This is a Tribbles Homolog 3 (Drosophila) plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2210 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3387440
Supplier Product No.: sc112991
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TRIB3 cDNA Clone in Mammalian Expression Vector (ABIN3387440)
Gene
TRIB3
(Tribbles Homolog 3 (Drosophila) (TRIB3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc112991
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TRIB3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2210 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Cellular stress induces TRB3/USP9x-dependent Notch activation in cancer." in: Oncogene, (2016) (PubMed).
: "High throughput kinase inhibitor screens reveal TRB3 and MAPK-ERK/TGFβ pathways as fundamental Notch regulators in breast cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 5, pp. 1714-9, (2013) (PubMed).
: "Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression." in: The EMBO journal, Vol. 29, Issue 3, pp. 559-73, (2010) (PubMed).
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: "Cellular stress induces TRB3/USP9x-dependent Notch activation in cancer." in: Oncogene, (2016) (PubMed).
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- TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))
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Alternative Name
- TRIB3
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Background
- The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014].Transcript Variant: This variant (1, also known as 1A) represents the longest transcript and encode the predominant isoform (1).
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NCBI Accession
- NM_021158, NP_066981
Target
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