Human TSG101 cDNA Clone in Mammalian Expression Vector
This is a Tumor Susceptibility Gene 101 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1560 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3387479
Supplier Product No.: sc116200
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TSG101 cDNA Clone in Mammalian Expression Vector (ABIN3387479)
Gene
TSG101
(Tumor Susceptibility Gene 101 (TSG101))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116200
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TSG101 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1560 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Engineering a synthetic cell panel to identify signalling components reprogrammed by the cell growth regulator anterior gradient-2." in: Molecular bioSystems, Vol. 10, Issue 6, pp. 1409-25, (2014) (PubMed).
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: "Engineering a synthetic cell panel to identify signalling components reprogrammed by the cell growth regulator anterior gradient-2." in: Molecular bioSystems, Vol. 10, Issue 6, pp. 1409-25, (2014) (PubMed).
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- TSG101 (Tumor Susceptibility Gene 101 (TSG101))
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Alternative Name
- TSG101
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Background
- The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_006292, NP_006283
Target
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