Human TUBB3 cDNA Clone in Mammalian Expression Vector
This is a Tubulin, beta 3 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1770 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3387517
Supplier Product No.: sc116313
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TUBB3 cDNA Clone in Mammalian Expression Vector (ABIN3387517)
Gene
TUBB3
(Tubulin, beta 3 (TUBB3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116313
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TUBB3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1770 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "EpCAM-selective elimination of carcinoma cells by a novel MAP-based cytolytic fusion protein." in: Molecular cancer therapeutics, Vol. 13, Issue 9, pp. 2194-202, (2014) (PubMed).
: "Strategies for diminishing katanin-based loss of microtubules in tauopathic neurodegenerative diseases." in: Human molecular genetics, Vol. 20, Issue 4, pp. 763-78, (2011) (PubMed).
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- TUBB3 (Tubulin, beta 3 (TUBB3))
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Alternative Name
- TUBB3
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Background
- This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010].Transcript Variant: This variant (1) encodes the longest isoform (1).
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NCBI Accession
- NM_006086, NP_006077
Target
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