Human UBE2I cDNA Clone in Mammalian Expression Vector
This is a Ubiquitin-Conjugating Enzyme E2I plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1080 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3387575
Supplier Product No.: sc109867
$148.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human UBE2I cDNA Clone in Mammalian Expression Vector (ABIN3387575)
Gene
UBE2I
(Ubiquitin-Conjugating Enzyme E2I (UBE2I))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc109867
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human UBE2I is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1080 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "SUMO downregulates GLP-1-stimulated cAMP generation and insulin secretion." in: American journal of physiology. Endocrinology and metabolism, Vol. 302, Issue 6, pp. E714-23, (2012) (PubMed).
: "Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14830-41, (2011) (PubMed).
: "SUMOylation regulates Kv2.1 and modulates pancreatic beta-cell excitability." in: Journal of cell science, Vol. 122, Issue Pt 6, pp. 775-9, (2009) (PubMed).
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: "SUMO downregulates GLP-1-stimulated cAMP generation and insulin secretion." in: American journal of physiology. Endocrinology and metabolism, Vol. 302, Issue 6, pp. E714-23, (2012) (PubMed).
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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Alternative Name
- UBE2I
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Background
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the predominant transcript. Variants 1-4 encode the same protein.
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NCBI Accession
- NM_003345, NP_003336
Target
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