Human VAPB cDNA Clone in Mammalian Expression Vector
This is a VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2440 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3387670
Supplier Product No.: sc117187
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human VAPB cDNA Clone in Mammalian Expression Vector (ABIN3387670)
Gene
VAPB
(VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117187
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human VAPB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2440 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Amyotrophic lateral sclerosis-related VAPB P56S mutation differentially affects the function and survival of corticospinal and spinal motor neurons." in: Human molecular genetics, Vol. 22, Issue 21, pp. 4293-305, (2013) (PubMed).
: "VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis." in: Human molecular genetics, Vol. 21, Issue 6, pp. 1299-311, (2012) (PubMed).
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: "Amyotrophic lateral sclerosis-related VAPB P56S mutation differentially affects the function and survival of corticospinal and spinal motor neurons." in: Human molecular genetics, Vol. 22, Issue 21, pp. 4293-305, (2013) (PubMed).
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- VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))
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Alternative Name
- VAPB
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Background
- The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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NCBI Accession
- NM_004738, NP_004729
Target
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