Human ADCY6 cDNA Clone in Mammalian Expression Vector
This is a Adenylate Cyclase 6 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3388118
Supplier Product No.: sc126190
$1,510.74
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human ADCY6 cDNA Clone in Mammalian Expression Vector (ABIN3388118)
Gene
ADCY6
(Adenylate Cyclase 6 (ADCY6))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc126190
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ADCY6 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Store-operated cyclic AMP signalling mediated by STIM1." in: Nature cell biology, Vol. 11, Issue 4, pp. 433-42, (2009) (PubMed).
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: "Store-operated cyclic AMP signalling mediated by STIM1." in: Nature cell biology, Vol. 11, Issue 4, pp. 433-42, (2009) (PubMed).
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- ADCY6 (Adenylate Cyclase 6 (ADCY6))
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Alternative Name
- ADCY6
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Background
- This gene encodes a member of the adenylyl cyclase family of proteins, which are required for the synthesis of cyclic AMP. All members of this family have an intracellular N-terminus, a tandem repeat of six transmembrane domains separated by a cytoplasmic loop, and a C-terminal cytoplasmic domain. The two cytoplasmic regions bind ATP and form the catalytic core of the protein. Adenylyl cyclases are important effectors of transmembrane signaling pathways and are regulated by the activity of G protein coupled receptor signaling. This protein belongs to a small subclass of adenylyl cyclase proteins that are functionally related and are inhibited by protein kinase A, calcium ions and nitric oxide. A mutation in this gene is associated with arthrogryposis multiplex congenita. [provided by RefSeq, May 2015].Transcript Variant: This variant (1) has an alternate 5' UTR and contains an in-frame fragment of 159 bp in the coding region, as compared to variant 2. Isoform a encoded by this variant thus has the same N- and C- termini as isoform b encoded by variant 2, but is 53 aa longer.
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NCBI Accession
- NM_015270, NP_056085
Target
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