Human APBB2 cDNA Clone in Mammalian Expression Vector
This is a Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 2 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3388235
Supplier Product No.: sc327396
$326.70
Plus shipping costs $50.00
10 μg
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Quick Overview for Human APBB2 cDNA Clone in Mammalian Expression Vector (ABIN3388235)
Gene
APBB2
(Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 2 (APBB2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc327396
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human APBB2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- APBB2 (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 2 (APBB2))
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Alternative Name
- APBB2
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Background
- The protein encoded by this gene interacts with the cytoplasmic domains of amyloid beta (A4) precursor protein and amyloid beta (A4) precursor-like protein 2. This protein contains two phosphotyrosine binding (PTB) domains, which are thought to function in signal transduction. Polymorphisms in this gene have been associated with Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].Transcript Variant: This variant (6) differs in the presence and absence of exons in the 5' UTR and 5' coding region, and uses a downstream translational start codon, compared to variant 1. The resulting isoform (d) is significantly shorter at the N-terminus, compared to isoform a. Variants 4, 5, 6 and 7 encode the same isoform.
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NCBI Accession
- NM_001166053, NP_001159525
Target
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