Human ARRB1 cDNA Clone in Mammalian Expression Vector
This is a Arrestin, beta 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3388298
Supplier Product No.: sc303424
$679.14
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human ARRB1 cDNA Clone in Mammalian Expression Vector (ABIN3388298)
Gene
beta Arrestin 1 (ARRB1)
(Arrestin, beta 1 (ARRB1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc303424
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ARRB1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "β2 adrenergic receptor fluorescent protein fusions traffic to the plasma membrane and retain functionality." in: PLoS ONE, Vol. 8, Issue 9, pp. e74941, (2013) (PubMed).
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: "β2 adrenergic receptor fluorescent protein fusions traffic to the plasma membrane and retain functionality." in: PLoS ONE, Vol. 8, Issue 9, pp. e74941, (2013) (PubMed).
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- beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))
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Alternative Name
- ARRB1
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Background
- Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011].Transcript Variant: This variant (1) represents the longer transcript variant and encodes the longer isoform (A).
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NCBI Accession
- NM_004041, NP_004032
Target
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