Human ATP7B cDNA Clone in Mammalian Expression Vector
This is a ATPase, Cu++ Transporting, beta Polypeptide plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 6500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3388351
Supplier Product No.: sc308720
$1,241.46
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human ATP7B cDNA Clone in Mammalian Expression Vector (ABIN3388351)
Gene
ATP7B
(ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc308720
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ATP7B is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 6500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Involvement of protein kinase D in expression and trafficking of ATP7B (copper ATPase)." in: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7389-96, (2011) (PubMed).
: "High yield heterologous expression of wild-type and mutant Cu+-ATPase (ATP7B, Wilson disease protein) for functional characterization of catalytic activity and serine residues undergoing ..." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21307-16, (2009) (PubMed).
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- ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))
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Alternative Name
- ATP7B
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Background
- This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (a).
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NCBI Accession
- NM_000053, NP_000044
Target
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