Human AXL cDNA Clone in Mammalian Expression Vector
This is a AXL Receptor tyrosine Kinase plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3388375
Supplier Product No.: sc112559
$810.81
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human AXL cDNA Clone in Mammalian Expression Vector (ABIN3388375)
Gene
AXL
(AXL Receptor tyrosine Kinase (AXL))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc112559
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human AXL is ideal for over-expression of native protein for functional studies.
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "Aptamer-miRNA-212 Conjugate Sensitizes NSCLC Cells to TRAIL." in: Molecular therapy. Nucleic acids, Vol. 5, pp. e289, (2016) (PubMed).
: "Functional consequences of AXL sequence variants in hypogonadotropic hypogonadism." in: The Journal of clinical endocrinology and metabolism, Vol. 99, Issue 4, pp. 1452-60, (2014) (PubMed).
: "AXL receptor kinase is a mediator of YAP-dependent oncogenic functions in hepatocellular carcinoma." in: Oncogene, Vol. 30, Issue 10, pp. 1229-40, (2011) (PubMed).
-
: "Aptamer-miRNA-212 Conjugate Sensitizes NSCLC Cells to TRAIL." in: Molecular therapy. Nucleic acids, Vol. 5, pp. e289, (2016) (PubMed).
-
- AXL (AXL Receptor tyrosine Kinase (AXL))
-
Alternative Name
- AXL
-
Background
- The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013].Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
-
NCBI Accession
- NM_021913, NP_068713
Target
-
You are here:
