Human CYP2E1 cDNA Clone in Mammalian Expression Vector
This is a Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3389203
Supplier Product No.: sc128274
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human CYP2E1 cDNA Clone in Mammalian Expression Vector (ABIN3389203)
Gene
CYP2E1
(Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc128274
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CYP2E1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Chlorzoxazone metabolism by porcine cytochrome P450 enzymes and the effect of cytochrome b5." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 38, Issue 5, pp. 857-62, (2010) (PubMed).
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: "Chlorzoxazone metabolism by porcine cytochrome P450 enzymes and the effect of cytochrome b5." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 38, Issue 5, pp. 857-62, (2010) (PubMed).
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- CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
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Alternative Name
- CYP2E1
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Background
- This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_000773, NP_000764
Target
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