Human EGR1 cDNA Clone in Mammalian Expression Vector
This is a Early Growth Response 1 plasmid from OriGene - 5 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(5 references)
Catalog No. ABIN3389417
Supplier Product No.: sc128132
$751.41
Plus shipping costs $50.00
10 μg
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Quick Overview for Human EGR1 cDNA Clone in Mammalian Expression Vector (ABIN3389417)
Gene
EGR1
(Early Growth Response 1 (EGR1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc128132
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human EGR1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1800 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "EGR-1/Bax pathway plays a role in vitamin E δ-tocotrienol-induced apoptosis in pancreatic cancer cells." in: The Journal of nutritional biochemistry, Vol. 26, Issue 8, pp. 797-807, (2015) (PubMed).
: "EGR1 supports the osteogenic differentiation of dental stem cells." in: International endodontic journal, Vol. 48, Issue 2, pp. 185-92, (2015) (PubMed).
: "Identification of a functional TPH1 polymorphism associated with irritable bowel syndrome bowel habit subtypes." in: The American journal of gastroenterology, Vol. 108, Issue 11, pp. 1766-74, (2013) (PubMed).
: "EGR-1 activation by EGF inhibits MMP-9 expression and lymphoma growth." in: Blood, Vol. 116, Issue 5, pp. 759-66, (2010) (PubMed).
: "Cellular immediate-early gene expression occurs kinetically upstream of Epstein-Barr virus bzlf1 and brlf1 following cross-linking of the B cell antigen receptor in the Akata Burkitt lymphoma cell ..." in: Journal of virology, Vol. 84, Issue 23, pp. 12405-18, (2010) (PubMed).
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- EGR1 (Early Growth Response 1 (EGR1))
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Alternative Name
- EGR1
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Background
- The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014].
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NCBI Accession
- NM_001964, NP_001955
Target
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