Human HNF4A cDNA Clone in Mammalian Expression Vector
This is a Hepatocyte Nuclear Factor 4, alpha plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1400 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3390115
Supplier Product No.: sc307232
$679.14
Plus shipping costs $50.00
10 μg
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Quick Overview for Human HNF4A cDNA Clone in Mammalian Expression Vector (ABIN3390115)
Gene
HNF4A
(Hepatocyte Nuclear Factor 4, alpha (HNF4A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc307232
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HNF4A is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1400 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Hepatocyte nuclear factor-4{alpha} regulates the human organic anion transporter 1 gene in the kidney." in: American journal of physiology. Renal physiology, Vol. 292, Issue 6, pp. F1819-26, (2007) (PubMed).
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: "Hepatocyte nuclear factor-4{alpha} regulates the human organic anion transporter 1 gene in the kidney." in: American journal of physiology. Renal physiology, Vol. 292, Issue 6, pp. F1819-26, (2007) (PubMed).
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- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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Alternative Name
- HNF4A
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Background
- The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012].Transcript Variant: This variant (1) uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (HNF4alpha1) lacks a 10 aa protein segment compared to isoform HNF4alpha2.
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NCBI Accession
- NM_178849, NP_849180
Target
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