Human KCNK18 cDNA Clone in Mammalian Expression Vector
This is a Potassium Channel Subfamily K Member 18 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1200 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3390376
Supplier Product No.: sc307384
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 8 Business Days
Quick Overview for Human KCNK18 cDNA Clone in Mammalian Expression Vector (ABIN3390376)
Gene
KCNK18
(Potassium Channel Subfamily K Member 18 (KCNK18))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc307384
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human KCNK18 is ideal for over-expression of native protein for functional studies.
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Insert Length
- 1200 bp
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "Identification of novel small molecule modulators of K2P18.1 two-pore potassium channel." in: European journal of pharmacology, Vol. 740, pp. 603-10, (2014) (PubMed).
: "Noradrenergic depression of neuronal excitability in the entorhinal cortex via activation of TREK-2 K+ channels." in: The Journal of biological chemistry, Vol. 284, Issue 16, pp. 10980-91, (2009) (PubMed).
-
: "Identification of novel small molecule modulators of K2P18.1 two-pore potassium channel." in: European journal of pharmacology, Vol. 740, pp. 603-10, (2014) (PubMed).
-
- KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))
-
Alternative Name
- KCNK18
-
Background
- Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and the encoded protein functions as an outward rectifying potassium channel. A mutation in this gene has been found to be associated with migraine with aura.[provided by RefSeq, Jan 2011].
-
NCBI Accession
- NM_181840, NP_862823
Target
-
You are here:
