Human KDM4C cDNA Clone in Mammalian Expression Vector
This is a Lysine (K)-Specific Demethylase 4C plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3390398
Supplier Product No.: sc327228
$833.58
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 36 to 37 Business Days
Quick Overview for Human KDM4C cDNA Clone in Mammalian Expression Vector (ABIN3390398)
Gene
KDM4C
(Lysine (K)-Specific Demethylase 4C (KDM4C))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc327228
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human KDM4C is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))
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Alternative Name
- KDM4C
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Background
- This gene is a member of the Jumonji domain 2 (JMJD2) family. The encoded protein is a trimethylation-specific demethylase, and converts specific trimethylated histone residues to the dimethylated form. This enzymatic action regulates gene expression and chromosome segregation. Chromosomal aberrations and changes in expression of this gene may be found in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015].Transcript Variant: This variant (4) contains multiple differences in the 5' and 3' regions compared to variant 1, including initiation of translation at an alternate start codon. The resulting protein (isoform 4) has a longer N-terminus, a distinct C-terminus, and is shorter than isoform 1.
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NCBI Accession
- NM_001146696, NP_001140168
Target
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