Human MET cDNA Clone in Mammalian Expression Vector
This is a Met Proto-Oncogene plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 5000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3390801
Supplier Product No.: sc316318
$1,766.16
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MET cDNA Clone in Mammalian Expression Vector (ABIN3390801)
Gene
c-MET (MET)
(Met Proto-Oncogene (MET))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc316318
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MET is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "NPS-1034, a novel MET inhibitor, inhibits the activated MET receptor and its constitutively active mutants." in: Investigational new drugs, Vol. 32, Issue 3, pp. 389-99, (2014) (PubMed).
: "Insights into ALK-driven cancers revealed through development of novel ALK tyrosine kinase inhibitors." in: Cancer research, Vol. 71, Issue 14, pp. 4920-31, (2011) (PubMed).
: "MET receptor sequence variants R970C and T992I lack transforming capacity." in: Cancer research, Vol. 70, Issue 15, pp. 6233-7, (2010) (PubMed).
: "Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates." in: The EMBO journal, Vol. 27, Issue 5, pp. 804-16, (2008) (PubMed).
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: "NPS-1034, a novel MET inhibitor, inhibits the activated MET receptor and its constitutively active mutants." in: Investigational new drugs, Vol. 32, Issue 3, pp. 389-99, (2014) (PubMed).
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- c-MET (MET) (Met Proto-Oncogene (MET))
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Alternative Name
- MET
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Background
- The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
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NCBI Accession
- NM_000245, NP_000236
Target
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