Human MTRR cDNA Clone in Mammalian Expression Vector
This is a 5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3390983
Supplier Product No.: sc314547
$658.35
Plus shipping costs $50.00
10 μg
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Quick Overview for Human MTRR cDNA Clone in Mammalian Expression Vector (ABIN3390983)
Gene
MTRR
(5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase (MTRR))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc314547
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MTRR is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- MTRR (5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase (MTRR))
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Alternative Name
- MTRR
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Background
- This gene encodes a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. This protein functions in the synthesis of methionine by regenerating methionine synthase to a functional state. Because methionine synthesis requires methyl-group transfer by a folate donor, activity of the encoded enzyme is important for folate metabolism and cellular methylation. Mutations in this gene can cause homocystinuria-megaloblastic anemia, cbl E type. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015].Transcript Variant: This variant (2) has additional sequence at the 5' end of exon 1 and lacks sequence at the 3' end of exon 1, as compared to transcript variant 1. This results in an additional 27 aa at the N-terminus of the protein compared to isoform 1 encoded by transcript variant 1, the remainder of the protein remains in frame.
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NCBI Accession
- NM_024010, NP_076915
Target
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