Human NCOA3 cDNA Clone in Mammalian Expression Vector
This is a Nuclear Receptor Coactivator 3 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 7400 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3391054
Supplier Product No.: sc316311
$1,804.77
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human NCOA3 cDNA Clone in Mammalian Expression Vector (ABIN3391054)
Gene
NCOA3
(Nuclear Receptor Coactivator 3 (NCOA3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc316311
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NCOA3 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 7400 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))
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Alternative Name
- NCOA3
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Background
- The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010].Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1, resulting in a shorter isoform (b) compared to isoform a.
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NCBI Accession
- NM_006534, NP_006525
Target
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