Human NEDD4 cDNA Clone in Mammalian Expression Vector
This is a Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 5900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3391075
Supplier Product No.: sc310054
$816.75
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human NEDD4 cDNA Clone in Mammalian Expression Vector (ABIN3391075)
Gene
NEDD4
(Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc310054
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NEDD4 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5900 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "p34 is a novel regulator of the oncogenic behavior of NEDD4-1 and PTEN." in: Cell death and differentiation, Vol. 21, Issue 1, pp. 146-60, (2013) (PubMed).
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- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
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Alternative Name
- NEDD4
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Background
- Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple alternate 5' coding exons, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (1) is shorter and has a distinct N-terminus, compared to isoform 2.
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NCBI Accession
- NM_006154, NP_006145
Target
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