Human NLRP1 cDNA Clone in Mammalian Expression Vector
This is a NLR Family, Pyrin Domain Containing 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 4600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3391130
Supplier Product No.: sc308738
$1,248.39
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human NLRP1 cDNA Clone in Mammalian Expression Vector (ABIN3391130)
Gene
NLRP1
(NLR Family, Pyrin Domain Containing 1 (NLRP1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc308738
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NLRP1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "NLRP1 haplotypes associated with vitiligo and autoimmunity increase interleukin-1? processing via the NLRP1 inflammasome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 8, pp. 2952-6, (2013) (PubMed).
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- NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))
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Alternative Name
- NLRP1
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Background
- This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) encodes the longest isoform (1).
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NCBI Accession
- NM_033004, NP_127497
Target
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