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Human NLRP5 cDNA Clone in Mammalian Expression Vector

This is a NLR Family, Pyrin Domain Containing 5 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3500 bp. Transient expression. Suitable for PExp. Bacterial selection: Ampicillin.
OriGene
Catalog No. ABIN3391139
Supplier Product No.: sc306608
$1,527.57
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 4 to 9 Business Days

Quick Overview for Human NLRP5 cDNA Clone in Mammalian Expression Vector (ABIN3391139)

Gene

NLRP5 (NLR Family, Pyrin Domain Containing 5 (NLRP5))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-XL5

Promoter

Enhanced CMV Promoter, T7 Promoter

Bacterial Resistance

Ampicillin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc306608

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human NLRP5 is ideal for over-expression of native protein for functional studies.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    3500 bp

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Brozzetti, Alimohammadi, Morelli, Minarelli, Hallgren, Giordano, De Bellis, Perniola, Kämpe, Falorni: "Autoantibody response against NALP5/MATER in primary ovarian insufficiency and in autoimmune Addison's disease." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 5, pp. 1941-8, (2015) (PubMed).

    Tomar, Kaushal, Das, Gupta, Betterle, Goswami: "Prevalence and significance of NALP5 autoantibodies in patients with idiopathic hypoparathyroidism." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 4, pp. 1219-26, (2012) (PubMed).

  • Target

    NLRP5 (NLR Family, Pyrin Domain Containing 5 (NLRP5))

    Alternative Name

    NLRP5

    Background

    The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). Expression of this gene is restricted to the oocyte. A mouse gene that encodes a maternal oocyte protein, similar to this encoded protein, is required for normal early embryogenesis. [provided by RefSeq, Jul 2008].

    NCBI Accession

    NM_153447, NP_703148
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