Human NLRP5 cDNA Clone in Mammalian Expression Vector
This is a NLR Family, Pyrin Domain Containing 5 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3391140
Supplier Product No.: sc318548
$1,552.32
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human NLRP5 cDNA Clone in Mammalian Expression Vector (ABIN3391140)
Gene
NLRP5
(NLR Family, Pyrin Domain Containing 5 (NLRP5))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc318548
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NLRP5 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Autoantibody response against NALP5/MATER in primary ovarian insufficiency and in autoimmune Addison's disease." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 5, pp. 1941-8, (2015) (PubMed).
: "Autoimmune polyendocrine syndrome type 1 and NALP5, a parathyroid autoantigen." in: The New England journal of medicine, Vol. 358, Issue 10, pp. 1018-28, (2008) (PubMed).
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- NLRP5 (NLR Family, Pyrin Domain Containing 5 (NLRP5))
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Alternative Name
- NLRP5
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Background
- The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). Expression of this gene is restricted to the oocyte. A mouse gene that encodes a maternal oocyte protein, similar to this encoded protein, is required for normal early embryogenesis. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_153447, NP_703148
Target
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