Human RASGRP3 cDNA Clone in Mammalian Expression Vector
This is a RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3391813
Supplier Product No.: sc327632
$688.05
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human RASGRP3 cDNA Clone in Mammalian Expression Vector (ABIN3391813)
Gene
RASGRP3
(RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc327632
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RASGRP3 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- RASGRP3 (RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3))
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Alternative Name
- RASGRP3
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Background
- Members of the RAS (see HRAS, MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008].Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2) which is 1 aa shorter than isoform 1.
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NCBI Accession
- NM_015376, NP_056191
Target
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