Human ROCK1 cDNA Clone in Mammalian Expression Vector
This is a rho-Associated, Coiled-Coil Containing Protein Kinase 1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 6000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3391947
Supplier Product No.: sc124118
$1,720.62
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human ROCK1 cDNA Clone in Mammalian Expression Vector (ABIN3391947)
Gene
ROCK1
(rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc124118
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human ROCK1 is ideal for over-expression of native protein for functional studies.
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Insert Length
- 6000 bp
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "MiR-186 targets ROCK1 to suppress the growth and metastasis of NSCLC cells." in: Tumour biology, Vol. 35, Issue 9, pp. 8933-7, (2014) (PubMed).
: "miR-124 inhibits growth and invasion of gastric cancer by targeting ROCK1." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 15, Issue 16, pp. 6543-6, (2014) (PubMed).
-
: "MiR-186 targets ROCK1 to suppress the growth and metastasis of NSCLC cells." in: Tumour biology, Vol. 35, Issue 9, pp. 8933-7, (2014) (PubMed).
-
- ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
-
Alternative Name
- ROCK1
-
Background
- This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015].
-
NCBI Accession
- NM_005406, NP_005397
Target
-
You are here:
