Human SH2B1 cDNA Clone in Mammalian Expression Vector
This is a SH2B Adaptor Protein 1 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3392160
Supplier Product No.: sc326606
$608.85
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human SH2B1 cDNA Clone in Mammalian Expression Vector (ABIN3392160)
Gene
SH2B1
(SH2B Adaptor Protein 1 (SH2B1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc326606
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SH2B1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2800 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- SH2B1 (SH2B Adaptor Protein 1 (SH2B1))
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Alternative Name
- SH2B1
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Background
- This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009].Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon compared to variant 1. The resulting protein (isoform 2), also known as SH2B adaptor protein 1 beta, is shorter and has a distinct C-terminus compared to isoform 1. Variants 2, 3 and 4 encode the same protein.
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NCBI Accession
- NM_001145812, NP_001139284
Target
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