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Human SIRPG cDNA Clone in Mammalian Expression Vector

cDNA PExp Mammalian Expression Vector pCMV6-XL5 Ampicillin Enhanced CMV Promoter unconjugated Transient
Catalog No. ABIN3392202

Quick Overview for Human SIRPG cDNA Clone in Mammalian Expression Vector (ABIN3392202)

Gene

SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-XL5

Promoter

Enhanced CMV Promoter, T7 Promoter

Bacterial Resistance

Ampicillin

Expression Type

Transient
  • Species

    Human

    Purpose

    Untagged full-length cDNA clone from Human SIRPG is ideal for over-expression of native protein for functional studies.

    Brand

    TrueClones®

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    1071 bp

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Target

    SIRPG (Signal-Regulatory Protein gamma (SIRPG))

    Alternative Name

    SIRPG

    Background

    The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (2) lacks two internal segments within the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

    NCBI Accession

    NM_080816, NP_543006
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