Human SLCO1A2 cDNA Clone in Mammalian Expression Vector
This is a Solute Carrier Organic Anion Transporter Family, Member 1A2 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2240 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3392340
Supplier Product No.: sc309088
$607.86
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human SLCO1A2 cDNA Clone in Mammalian Expression Vector (ABIN3392340)
Gene
SLCO1A2
(Solute Carrier Organic Anion Transporter Family, Member 1A2 (SLCO1A2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc309088
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLCO1A2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2240 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Distinct interaction of nilotinib and imatinib with P-Glycoprotein in intracellular accumulation and cytotoxicity in CML Cell Line K562 cells." in: Biological & pharmaceutical bulletin, Vol. 37, Issue 8, pp. 1330-5, (2014) (PubMed).
: "Interaction of macrolide antibiotics with intestinally expressed human and rat organic anion-transporting polypeptides." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 37, Issue 12, pp. 2375-82, (2009) (PubMed).
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- SLCO1A2 (Solute Carrier Organic Anion Transporter Family, Member 1A2 (SLCO1A2))
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Alternative Name
- SLCO1A2
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Background
- This gene encodes a sodium-independent transporter which mediates cellular uptake of organic ions in the liver. Its substrates include bile acids, bromosulphophthalein, and some steroidal compounds. The protein is a member of the SLC21A family of solute carriers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2008].Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. The translation of this protein may be modulated by multiple upstream ORFs that are present in the 5' UTR.
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NCBI Accession
- NM_134431, NP_602307
Target
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