Human SMO cDNA Clone in Mammalian Expression Vector
This is a Smoothened Homolog (Drosophila) plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3392360
Supplier Product No.: sc122724
$1,071.18
Plus shipping costs $50.00
10 μg
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Quick Overview for Human SMO cDNA Clone in Mammalian Expression Vector (ABIN3392360)
Gene
SMO
(Smoothened Homolog (Drosophila) (SMO))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc122724
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SMO is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3800 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Identification of recurrent SMO and BRAF mutations in ameloblastomas." in: Nature genetics, Vol. 46, Issue 7, pp. 722-5, (2014) (PubMed).
: "Structure of the human smoothened receptor bound to an antitumour agent." in: Nature, Vol. 497, Issue 7449, pp. 338-43, (2013) (PubMed).
: "Structural insights into the role of the Smoothened cysteine-rich domain in Hedgehog signalling." in: Nature communications, Vol. 4, pp. 2965, (2013) (PubMed).
: "Selective down-regulation of glioma-associated oncogene 2 inhibits the proliferation of hepatocellular carcinoma cells." in: Cancer research, Vol. 67, Issue 8, pp. 3583-93, (2007) (PubMed).
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- SMO (Smoothened Homolog (Drosophila) (SMO))
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Alternative Name
- SMO
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Background
- The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010].
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NCBI Accession
- NM_005631, NP_005622
Target
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