Human Stim2 cDNA Clone in Mammalian Expression Vector
This is a Stromal Interaction Molecule 2 plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3392504
Supplier Product No.: sc314792
$756.36
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human Stim2 cDNA Clone in Mammalian Expression Vector (ABIN3392504)
Gene
Stim2
(Stromal Interaction Molecule 2 (Stim2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc314792
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human STIM2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3900 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Stromal interaction molecule (STIM) 1 and STIM2 calcium sensing regions exhibit distinct unfolding and oligomerization kinetics." in: The Journal of biological chemistry, Vol. 284, Issue 2, pp. 728-32, (2009) (PubMed).
: "Store-operated cyclic AMP signalling mediated by STIM1." in: Nature cell biology, Vol. 11, Issue 4, pp. 433-42, (2009) (PubMed).
: "TRPC channels function independently of STIM1 and Orai1." in: The Journal of physiology, Vol. 587, Issue Pt 10, pp. 2275-98, (2009) (PubMed).
: "Role of the store-operated calcium entry proteins Stim1 and Orai1 in muscarinic cholinergic receptor-stimulated calcium oscillations in human embryonic kidney cells." in: The Journal of physiology, Vol. 579, Issue Pt 3, pp. 679-89, (2007) (PubMed).
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- Stim2 (Stromal Interaction Molecule 2 (Stim2))
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Alternative Name
- STIM2
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Background
- This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009].Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.
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NCBI Accession
- NM_020860, NP_065911
Target
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