Human U2AF59 cDNA Clone in Mammalian Expression Vector
This is a U2 Small Nuclear RNA Auxiliary Factor 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2149 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3392950
Supplier Product No.: sc122300
$472.23
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human U2AF59 cDNA Clone in Mammalian Expression Vector (ABIN3392950)
Gene
U2AF2 (U2AF59)
(U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc122300
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human U2AF2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2149 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal." in: Nature communications, Vol. 7, pp. 10950, (2016) (PubMed).
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: "An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal." in: Nature communications, Vol. 7, pp. 10950, (2016) (PubMed).
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- U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))
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Alternative Name
- U2AF2
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Background
- U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008].
Target
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