Human ADAR cDNA Clone in Mammalian Expression Vector
This is a Adenosine Deaminase, RNA-Specific plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 7000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3393339
Supplier Product No.: sc119438
$1,585.98
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ADAR cDNA Clone in Mammalian Expression Vector (ABIN3393339)
Gene
ADAR
(Adenosine Deaminase, RNA-Specific (ADAR))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119438
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ADAR is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 7000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "PTBP1 induces ADAR1 p110 isoform expression through IRES-like dependent translation control and influences cell proliferation in gliomas." in: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 22, pp. 4383-97, (2015) (PubMed).
: "Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria." in: BMC medical genetics, Vol. 15, pp. 69, (2014) (PubMed).
: "F11R expression upon hypoxia is regulated by RNA editing." in: PLoS ONE, Vol. 8, Issue 10, pp. e77702, (2013) (PubMed).
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: "PTBP1 induces ADAR1 p110 isoform expression through IRES-like dependent translation control and influences cell proliferation in gliomas." in: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 22, pp. 4383-97, (2015) (PubMed).
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- ADAR (Adenosine Deaminase, RNA-Specific (ADAR))
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Alternative Name
- ADAR
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Background
- This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010].Transcript Variant: This variant (1) encodes the longest isoform (a, also referred to as ADAR-a).
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NCBI Accession
- NM_001111, NP_001102
Target
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