Human ARFGAP1 cDNA Clone in Mammalian Expression Vector
This is a ADP-Ribosylation Factor GTPase Activating Protein 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 2900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3393357
Supplier Product No.: sc110523
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ARFGAP1 cDNA Clone in Mammalian Expression Vector (ABIN3393357)
Gene
ARFGAP1
(ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc110523
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ARFGAP1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2900 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, (2007) (PubMed).
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: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, (2007) (PubMed).
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- ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))
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Alternative Name
- ARFGAP1
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Background
- The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].Transcript Variant: This variant (1) lacks an in-frame exon and uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. The encoded isoform (a) is shorter, compared to isoform b.
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NCBI Accession
- NM_018209, NP_060679
Target
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