Human CTDP1 cDNA Clone in Mammalian Expression Vector
This is a CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Phosphatase, Subunit 1 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 4000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3393449
Supplier Product No.: sc111100
$787.05
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human CTDP1 cDNA Clone in Mammalian Expression Vector (ABIN3393449)
Gene
CTDP1
(CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Phosphatase, Subunit 1 (CTDP1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc111100
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CTDP1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- CTDP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Phosphatase, Subunit 1 (CTDP1))
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Alternative Name
- CTDP1
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Background
- This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011].Transcript Variant: This variant (2) is missing a coding exon in the 3' region compared to variant 1. This results in a frame-shift and a shorter isoform (2, also known as FCP1b) with a distinct C-terminus (lacking the carboxy-terminal TFIIF-binding protein domain) compared to isoform 1.
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NCBI Accession
- NM_048368, NP_430255
Target
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