Human GATA6 cDNA Clone in Mammalian Expression Vector
This is a GATA Binding Protein 6 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 3100 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3393535
Supplier Product No.: sc116877
$823.68
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human GATA6 cDNA Clone in Mammalian Expression Vector (ABIN3393535)
Gene
GATA6
(GATA Binding Protein 6 (GATA6))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116877
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GATA6 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3100 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "A novel mutation in GATA6 causes pancreatic agenesis." in: Pediatric diabetes, Vol. 16, Issue 1, pp. 67-70, (2015) (PubMed).
: "Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAs." in: PLoS ONE, Vol. 9, Issue 6, pp. e100134, (2014) (PubMed).
: "GATA-6 and NF-κB activate CPI-17 gene transcription and regulate Ca2+ sensitization of smooth muscle contraction." in: Molecular and cellular biology, Vol. 33, Issue 5, pp. 1085-102, (2013) (PubMed).
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- GATA6 (GATA Binding Protein 6 (GATA6))
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Alternative Name
- GATA6
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Background
- This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012].
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NCBI Accession
- NM_005257, NP_005248
Target
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