Human GEM cDNA Clone in Mammalian Expression Vector
This is a GTP Binding Protein Overexpressed in Skeletal Muscle plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 2100 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3393536
Supplier Product No.: sc128239
$297.00
Plus shipping costs $50.00
10 μg
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Quick Overview for Human GEM cDNA Clone in Mammalian Expression Vector (ABIN3393536)
Gene
GEM
(GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc128239
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GEM is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2100 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Equine herpesvirus 1 enters cells by two different pathways, and infection requires the activation of the cellular kinase ROCK1." in: Journal of virology, Vol. 81, Issue 20, pp. 10879-89, (2007) (PubMed).
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: "Equine herpesvirus 1 enters cells by two different pathways, and infection requires the activation of the cellular kinase ROCK1." in: Journal of virology, Vol. 81, Issue 20, pp. 10879-89, (2007) (PubMed).
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- GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))
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Alternative Name
- GEM
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Background
- The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
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NCBI Accession
- NM_005261, NP_005252
Target
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