Human LAMP2 cDNA Clone in Mammalian Expression Vector
This is a Lysosomal-Associated Membrane Protein 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 4450 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3393595
Supplier Product No.: sc127527
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human LAMP2 cDNA Clone in Mammalian Expression Vector (ABIN3393595)
Gene
LAMP2
(Lysosomal-Associated Membrane Protein 2 (LAMP2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc127527
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human LAMP2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4450 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Chaperone-mediated autophagy regulates proliferation by targeting RND3 in gastric cancer." in: Autophagy, Vol. 12, Issue 3, pp. 515-28, (2016) (PubMed).
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: "Chaperone-mediated autophagy regulates proliferation by targeting RND3 in gastric cancer." in: Autophagy, Vol. 12, Issue 3, pp. 515-28, (2016) (PubMed).
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- LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))
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Alternative Name
- LAMP2
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Background
- The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (B) results from alternative splicing of exon 9 and results in a shorter transcript, but not protein, as compared to variant A. Transcript variant B is over-expressed in muscle and encodes isoform B.
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NCBI Accession
- NM_013995, NP_054701
Target
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