Human LEPROT cDNA Clone in Mammalian Expression Vector
cDNA
PExp
Mammalian Expression Vector
pCMV6-XL6
Ampicillin
Enhanced CMV Promoter
unconjugated
Transient
Catalog No. ABIN3393599
Quick Overview for Human LEPROT cDNA Clone in Mammalian Expression Vector (ABIN3393599)
Gene
LEPROT
(Leptin Receptor Overlapping Transcript (LEPROT))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Purpose
- Untagged full-length cDNA clone from Human LEPROT is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- LEPROT (Leptin Receptor Overlapping Transcript (LEPROT))
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Alternative Name
- LEPROT
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Background
- LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR, MIM 600946) and leptin receptor (OBR, or LEPR, MIM 601007), thereby altering receptor-mediated cell signaling (Couturier et al., 2007 [PubMed 18042720], Touvier et al., 2009 [PubMed 19907080]).[supplied by OMIM, Jul 2010].Transcript Variant: This variant (1) is dominant and encodes the shorter isoform (1).
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NCBI Accession
- NM_017526, NP_059996
Target
-
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