Human LRP5 cDNA Clone in Mammalian Expression Vector
This is a Low Density Lipoprotein Receptor-Related Protein 5 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 4700 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3393610
Supplier Product No.: sc118678
$1,368.18
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human LRP5 cDNA Clone in Mammalian Expression Vector (ABIN3393610)
Gene
LRP5
(Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118678
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human LRP5 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4700 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Identification of two novel LRP5 mutations in families with familial exudative vitreoretinopathy." in: Molecular vision, Vol. 20, pp. 395-409, (2014) (PubMed).
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: "Identification of two novel LRP5 mutations in families with familial exudative vitreoretinopathy." in: Molecular vision, Vol. 20, pp. 395-409, (2014) (PubMed).
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- LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
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Alternative Name
- LRP5
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Background
- This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014].Transcript Variant: This variant (1) encodes the longer isoform (1).
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NCBI Accession
- NM_002335, NP_002326
Target
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