Human NPR1 cDNA Clone in Mammalian Expression Vector
This is a Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 3940 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3393669
Supplier Product No.: sc125506
$914.76
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human NPR1 cDNA Clone in Mammalian Expression Vector (ABIN3393669)
Gene
NPR1
(Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) (NPR1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc125506
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NPR1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3940 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Knockdown of natriuretic peptide receptor-A enhances receptor C expression and signalling in vascular smooth muscle cells." in: Cardiovascular research, Vol. 93, Issue 2, pp. 350-9, (2012) (PubMed).
: "Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide." in: The Journal of endocrinology, Vol. 201, Issue 2, pp. 287-95, (2009) (PubMed).
: "Successive action of meprin A and neprilysin catabolizes B-type natriuretic peptide." in: Circulation research, Vol. 101, Issue 9, pp. 875-82, (2007) (PubMed).
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- NPR1 (Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) (NPR1))
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Alternative Name
- NPR1
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Background
- Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides, they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2, MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009].
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NCBI Accession
- NM_000906, NP_000897
Target
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