Human PDGFB cDNA Clone in Mammalian Expression Vector
This is a Platelet Derived Growth Factor Subunit B plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 2800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3393687
Supplier Product No.: sc111665
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PDGFB cDNA Clone in Mammalian Expression Vector (ABIN3393687)
Gene
PDGFB
(Platelet Derived Growth Factor Subunit B (PDGFB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc111665
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PDGFB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2800 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor." in: Biomaterials, Vol. 35, Issue 2, pp. 737-47, (2013) (PubMed).
: "Defective N-sulfation of heparan sulfate proteoglycans limits PDGF-BB binding and pericyte recruitment in vascular development." in: Genes & development, Vol. 21, Issue 3, pp. 316-31, (2007) (PubMed).
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: "The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor." in: Biomaterials, Vol. 35, Issue 2, pp. 737-47, (2013) (PubMed).
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- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
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Alternative Name
- PDGFB
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Background
- This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015].Transcript Variant: This variant (1) encodes the longer isoform (1) with a signal peptide.
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NCBI Accession
- NM_002608, NP_002599
Target
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