Human TAF1C cDNA Clone in Mammalian Expression Vector
This is a TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 4000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3393850
Supplier Product No.: sc110016
$703.89
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TAF1C cDNA Clone in Mammalian Expression Vector (ABIN3393850)
Gene
TAF1C
(TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa (TAF1C))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc110016
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TAF1C is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- TAF1C (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa (TAF1C))
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Alternative Name
- TAF1C
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Background
- Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011].Transcript Variant: This variant (2) has alternate splice sites in three regions, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, lacks an internal segment and an internal amino acid 'G', compared to isoform 1.
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NCBI Accession
- NM_139353, NP_647610
Target
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